Inhibitory Effect of EUTHYMOL Original Toothpaste on VSC-producing Oral Bacteria
김효진, 최원경, 이원경, 김원찬, 오경희, 하원호,
소속 상세정보
김효진 ( Kim Hyo-Jin ) - LG H&H Research Center Preventive Dentistry Research Team
최원경 ( Choi Won-Kyung ) - LG H&H Research Center Deodorizing Tech Project
이원경 ( Lee Won-Kyung ) - LG H&H Research Center Deodorizing Tech Project
김원찬 ( Kim Won-Chan ) - LG H&H Research Center Deodorizing Tech Project
오경희 ( Oh Kyoung-Hee ) - LG H&H Research Center Preventive Dentistry Research Team
하원호 ( Ha Won-Ho ) - LG H&H Research Center Oral Health R&D Division
Abstract
Objective: Oral malodor (bad breath) is generally the result of microbial metabolism, and volatile sulfur compounds (VSC) producing bacteria are mainly present in biofilm of tongue dorsum, are the main cause of halitosis. Many studies have revealed that these bacteria are also associated with periodontal disease. We developed a flavoring agent by combining ingredients that identified to have oral antibacterial properties, and applied to EUTHYMOL original toothpaste. In this study, we examine the effect of suppressing oral bacteria and reducing VSC production of the toothpaste.
Methods: 1) Oral bacteria inhibitory test: P. gingivalis, F. periodonticum are cultured in BHI medium at anaerobic incubation system. The toothpaste samples were treated for 24h on bacterium culture condition, VSC was detected by Oral Chroma. 2) Salivary incubation test: Saliva suspension from healthy adults was incubated anaerobically with amino acids (cysteine and methionine) and toothpaste samples for 4h, and VSC was measured by Oral Chroma.
Results: As a result of the VSC inhibition test of oral bacteria, 82.6% of P. gingivalis inhibition rate was revealed compared to the non-treated group, and 1.41-fold difference compared to the unscented toothpaste treatment group. Also, 49.2% of F. periodonticum inhibition rate was showed compared to the non-treated group. In saliva incubation test, the VSC inhibition rate was 58.9% compared to the non-treated group, and 1.45-fold difference compared to the unscented toothpaste treatment group.
Conclusion: We revealed the anti-microbial effect of EUTHYMOL original toothpaste on halitosis-inducing oral bacteria. Through this study, the effect of suppressing bad breath through VSC inhibition in oral cavity can be expected.
키워드
halitosis; VSC; bacteria; saliva; EUTHYMOL
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